Differences of shelf - life in some melons including six varieties of cucumis melo l 6个变种部分品种货架期差异比较
Cucumis melo l 来自甜瓜属
Cloning and sequencing of cucumis melo fruit - specific expression cucumisin gene promoter region 甜瓜果实特异性表达黄瓜素基因启动子区的克隆和序列分析
Genetic transformation of the acc oxidase antisense gene to cucumis melo l . cv . hetao via agrobacterium tumefaciens 氧化酶反义基因转化甜瓜品种河套蜜瓜的研究
Handline cucumis melo and aqualica forsk seeds with different concentrations of detergent and soap , shows germination of detergent and soap and that even the effect of the same concentration of detergent and soap on the seeds is different 摘要以不同浓度的洗衣粉、肥皂液处理空心菜、香瓜种子,发现不同浓度的洗衣粉,肥皂液均能不同程度抑制种子萌发及幼苗生长,且相同浓度洗衣粉、肥皂液对种子生长影响也存在差异。
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed 本实验以河套蜜瓜果肉基因组dna为模板,用甜瓜acc氧化酶基因特异寡核苷酸链为引物进行pcr扩增,得到128bp的扩增产物。将得到的扩增产物克隆到质粒载体pmd - 18 - t上,筛选反向克隆,然后将其反向构建到植物表达载体pbinyxw的camv35s启动子和tmv增强子“ ”的下游,构建成反义表达载体pbinya 。并在对河套蜜瓜授粉受精生物学研究的基础上,通过花粉管通道法转化河套蜜瓜,共获76颗瓜,并进行了硬度和含糖量的分析。